Immunohistochemistry Collagen VII antibody [LH7.2] Mouse Human

Get tips on using Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729) to perform ChIP Anti-bodies H3K27me3

Get tips on using Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729) to perform ChIP Anti-bodies H3K27ac

Get tips on using Active BDNF (Human, Rat) ELISA Kit to perform ELISA Mouse - GDNF

Get tips on using siGENOME Mouse Alox12 siRNA to perform siRNA / miRNA gene silencing Mouse - B16-F10 12-Lox/ALOX12

Get tips on using Recombinant Anti-alpha smooth muscle Actin antibody [E184] (ab32575) to perform Western blotting Smooth muscle actin

Get tips on using Anti-Histone H3 (acetyl K9) antibody - ChIP Grade (ab4441) to perform ChIP Anti-bodies H3K9-Ac

Get tips on using Anti-PI 3 Kinase p85 alpha (phospho Y607) antibody to perform Autophagy assay cell type - HepG2

Get tips on using Anti-PI3-kinase p85-α antibody produced in rabbit to perform Autophagy assay cell type - HepG2

Get tips on using Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade to perform ChIP H3K36Me3 - Sheep Rat -NA-

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.