Immunohistochemistry Anti-Glial Fibrillary Acidic Protein (GFAP) Mouse Human

The process of RNA extraction from bacteria, in general, involves an RNA-protective, effective lysis of bacterial cell wall (which may pose difficulties). EDTA promotes loss of outer membrane to provide lysozyme with access to peptidoglycan. Another common method for cell wall lysis is mechanical disruption using a homogenizer (applied for gram-positive bacteria and some strains of gram-negative bacteria). Following lysis, it is necessary to disrupt protein-nucleic acid interactions, which can be achieved by adding sodium dodecyl sulfate (SDS). Next step involves using phenol-chloroform-isoamyl alcohol extraction, where RNA can be obtained from the bottom organic phase, the top phase consists of DNA and the interphase contains proteins. Isoamyl alcohol is an inert and optional addition to this mixture and is added as an anti-foaming reagent to reduce the interphase. Following RNA extraction, the samples should be checked for its quality by gel electrophoresis (23S and 16S rRNAs and 5s rRNA and tRNA bands) or UV spectrophotometric or fluorescence methods.

Get tips on using Mouse GDNF ELISA Kit (ab171178) to perform ELISA Human - GDNF

Get tips on using Goat Anti-Rabbit IgG (H + L)-HRP Conjugate to perform Western blot Secondary Antibody - Goat Rabbit Horseradish peroxidase

Get tips on using Recombinant Anti-STAT3 (phospho S727) antibody [E121-31] (ab32143) to perform Western blotting STAT3

Get tips on using Recombinant Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (ab181537) to perform Western blotting Ubiquitin

Get tips on using Recombinant Anti-IKK alpha + IKK beta antibody [EPR16628] (ab178870) to perform Western blotting IKK Alpha

Get tips on using Recombinant Anti-Cyclin D1 antibody [EPR2241] - C-terminal (ab134175) to perform Western blotting Cyclin D1

Get tips on using Qproteome Nuclear Protein Kit to perform Protein enrichment Soluble nuclear proteins

Get tips on using Atg12 Antibody (Mouse Specific) #2011 to perform Autophagy assay cell type - MEFs (mouse embryonic fibroblasts)

Get tips on using Anti-PI 3 Kinase p85 alpha (phospho Y607) antibody to perform Autophagy assay cell type - HepG2