Site Directed Mutagenesis (SDM) Monkey Deletion

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human peripheral blood monocytes

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

Get tips on using BRAIN HEART INFUSION BROTH to perform Bacterial cell culture media Listeria monocytogens

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Bacteria - Gram positive Listeria monocytogens

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Bacteria - Gram positive Listeria monocytogens

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary human CD14+ monocytes

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary bovine monocyte derived macrophages

Get tips on using Qproteome Bacterial Protein Prep Kit to perform Protein isolation Bacteria - Listeria monocytogenes

Get tips on using BHI-BRAIN HEART INFUSION BROTH to perform Bacterial cell culture media Listeria monocytogens