Site Directed Mutagenesis (SDM) Monkey Deletion

Get tips on using TRIzol™ LS Reagent to perform RNA isolation / purification Bacteria - Gram positive Listeria monocytogens

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Cells - primary human CD14+ monocytes

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Cells - primary human peripheral blood monocytes

Get tips on using HiPerFect Transfection Reagent (100 ml) to perform siRNA / RNAi /miRNA transfection Bovine - monocyte-derived macrophages

Get tips on using AllPrep DNA/RNA Mini Kit to perform RNA isolation / purification Cells - primary human CD14+ monocytes

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

Get tips on using EasySep™ Human CD33 Positive Selection Kit II to perform Cell Isolation Monocyte

Get tips on using B-PER™ Bacterial Protein Extraction Reagent to perform Protein isolation Bacteria - Listeria monocytogenes

Get tips on using In Situ Cell Proliferation Kit, FLUOS to perform Cell cytotoxicity / Proliferation assay cell type - HUVEC