siRNA / miRNA gene silencing Mouse 3T3-SA

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

Get tips on using Mouse SDF1 ELISA Kit (ab100741) to perform ELISA Mouse - SDF-1/CXCL12

Get tips on using Anti-Mouse CD31 (PECAM-1) to perform Immunohistochemistry CD31 - Rat Mouse -NA-

Get tips on using Bicinchoninic Acid Kit for Protein Determination to perform Protein quantification Mammalian cells - 3T3

Get tips on using Micro BCA™ Protein Assay Kit to perform Protein quantification Mammalian cells - 3T3

Get tips on using PE Rat Anti-Mouse CD103 to perform Flow cytometry Anti-bodies Mouse - CD103

Get tips on using APC anti-mouse CD103 Antibody to perform Flow cytometry Anti-bodies Mouse - CD103

Get tips on using Purified anti-mouse CD68 Antibody to perform Flow cytometry Anti-bodies Mouse - CD68

Get tips on using APC anti-mouse CD68 Antibody to perform Flow cytometry Anti-bodies Mouse - CD68

Get tips on using FITC anti-mouse CD25 Antibody to perform Flow cytometry Anti-bodies Mouse - CD25