DNA transfection Mammalian cells Immortalized cell lines

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Get tips on using GenElute™ Mammalian Total RNA Miniprep Kit to perform

Products Sigma-Aldrich GenElute™ Mammalian Total RNA Miniprep Kit

Get tips on using pcDNA™3.1 (+) Mammalian Expression Vector to perform siRNA / miRNA gene silencing Human - U251 cofilin-1 (CFL1)

Products Thermo Fisher Scientific pcDNA™3.1 (+) Mammalian Expression Vector

Wound healing assay can be challenging due to inconsistencies and variations while making a wound on the confluent cell monolayer, consequently leads to wounds of varying sizes and widths. Moreover, this assay causes damage to the cells that are at the edge of the wound, which can prevent cell migration into the wound site and healing. The best solution is to use the standard wound healing assay kits using either combs or inserts to make a defined wound field or gap and prevent the well-to-well variation in these assays.

Cellular assays Wound healing assay cell type human gHMVEC (glioma human microvascular endothelial cells)

Get tips on using TIANamp Genomic DNA Kit to perform DNA isolation / purification Cells - Primary cells Mouse embryonic fibroblast (MEF)

Products Tiangen TIANamp Genomic DNA Kit

Get tips on using Wizard® Genomic DNA Purification Kit to perform DNA isolation / purification Cells - Primary cells HUVEC

Products Promega Wizard® Genomic DNA Purification Kit

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized HSG cells

Products Thermo Fisher Scientific TRIzol Reagent

Get tips on using Endothelial Cell Medium to perform Mammalian cell culture media CADMEC/HMVEC

Products ScienCell Research Laboratories Endothelial Cell Medium

Get tips on using Vascular Cell Basal Medium to perform Mammalian cell culture media HCAEC

Products LGC Standards Vascular Cell Basal Medium

Get tips on using Smooth Muscle Cell Medium to perform Mammalian cell culture media HAOSMC

Products ScienCell Research Laboratories Smooth Muscle Cell Medium

Get tips on using Canine Endothelial Cell Media to perform Mammalian cell culture media CnAOEC

Products Cell Applications Inc Canine Endothelial Cell Media

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