Get tips on using Human Dkk-1 ELISA Kit (RAB0143) to perform ELISA Human - Dkk-1
TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.
Get tips on using Alexa Fluor® 647 Rat Anti-Mouse CD206 to perform Flow cytometry Anti-bodies Mouse - CD206
Get tips on using PE-Cy™7 Rat Anti-Mouse CD86 to perform Flow cytometry Anti-bodies Mouse - CD86
Get tips on using Alexa Fluor® 700 Rat Anti-Mouse CD86 to perform Flow cytometry Anti-bodies Mouse - CD86
Get tips on using PerCP-Cy™5.5 Rat Anti-Mouse CD19 to perform Flow cytometry Anti-bodies Mouse - CD19
Get tips on using PerCP-Cy™5.5 Rat Anti-Mouse CD8a to perform Flow cytometry Anti-bodies Mouse - CD8a
Get tips on using PerCP-Cy™5.5 Rat Anti-Mouse CD4 to perform Flow cytometry Anti-bodies Mouse - CD4
Get tips on using Alexa Fluor® 647 Rat anti-Mouse CD34 to perform Flow cytometry Anti-bodies Mouse - CD34
Get tips on using 1 Kb DNA Ladders to perform DNA Ladder 1 kb
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