siRNA / miRNA gene silencing Rat RMC-1

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Get tips on using Human Dkk-1 ELISA Kit (RAB0143) to perform ELISA Human - Dkk-1

Products Sigma-Aldrich Human Dkk-1 ELISA Kit (RAB0143)

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Cellular assays TUNEL assay cell type Rat fibroblast-like synoviocytes

Get tips on using Alexa Fluor® 647 Rat Anti-Mouse CD206 to perform Flow cytometry Anti-bodies Mouse - CD206

Products BD Biosciences Alexa Fluor® 647 Rat Anti-Mouse CD206

Get tips on using PE-Cy™7 Rat Anti-Mouse CD86 to perform Flow cytometry Anti-bodies Mouse - CD86

Products BD Biosciences PE-Cy™7 Rat Anti-Mouse CD86

Get tips on using Alexa Fluor® 700 Rat Anti-Mouse CD86 to perform Flow cytometry Anti-bodies Mouse - CD86

Products BD Biosciences Alexa Fluor® 700 Rat Anti-Mouse CD86

Get tips on using PerCP-Cy™5.5 Rat Anti-Mouse CD19 to perform Flow cytometry Anti-bodies Mouse - CD19

Products BD Biosciences PerCP-Cy™5.5 Rat Anti-Mouse CD19

Get tips on using PerCP-Cy™5.5 Rat Anti-Mouse CD8a to perform Flow cytometry Anti-bodies Mouse - CD8a

Products BD Biosciences PerCP-Cy™5.5 Rat Anti-Mouse CD8a

Get tips on using PerCP-Cy™5.5 Rat Anti-Mouse CD4 to perform Flow cytometry Anti-bodies Mouse - CD4

Products BD Biosciences PerCP-Cy™5.5 Rat Anti-Mouse CD4

Get tips on using Alexa Fluor® 647 Rat anti-Mouse CD34 to perform Flow cytometry Anti-bodies Mouse - CD34

Products BD Biosciences Alexa Fluor® 647 Rat anti-Mouse CD34

Get tips on using 1 Kb DNA Ladders to perform DNA Ladder 1 kb

Products Xcelris Genomics 1 Kb DNA Ladders

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