Protein quantification Mammalian cells

Get tips on using pAU5-amyE to perform Protein Expression Prokaryotic cells - C. glutamicum amyE

Get tips on using pBNS2-man to perform Protein Expression Prokaryotic cells - B. subtilis mannanase

Get tips on using pBYR2T-EGFP to perform Protein Expression Prokaryotic cells - A. tumefaciens GFP

Get tips on using pFastBac1-TEV-TRPV1 to perform Protein Expression Eukaryotic cells - HEK293 TRPV1

Get tips on using pCMV-HA2/DKK1 to perform Protein Expression Eukaryotic cells - HEK293 DKK1

Get tips on using pgMAX system-DsRed2 to perform Protein Expression Eukaryotic cells - HEK293 DsRed2

Get tips on using pCEP-4-MBP to perform Protein Expression Eukaryotic cells - HEK293 BM40

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

Get tips on using LIVE/DEAD™ Fixable Aqua Dead Cell Stain Kit to perform Live / Dead assay mammalian cells - mouse, T-cell

Get tips on using X-tremeGENE™ HP DNA Transfection Reagent to perform DNA transfection Mammalian cells - Immortalized cell lines MDA-MB-231