Protein expression and purification Mammalian cells HEK 293

DNA microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.

Get tips on using pTSara-NatB to perform Protein Expression Prokaryotic cells - E. coli N-terminal acetyltransferase B

Get tips on using pQE-30 to perform Protein Expression Prokaryotic cells - E. coli Guinea Pig TNF-Alpha

Get tips on using pET30a-β4 to perform Protein Expression Prokaryotic cells - E. coli E. granulosus β4 tubulin

Get tips on using pET30a-α9 to perform Protein Expression Prokaryotic cells - E. coli E. granulosus α9 tubulin

Get tips on using pFastBac-MRP4-his6 to perform Protein Expression Eukaryotic cells - S. frugiperda human MRP4-his6

Get tips on using pPICZαC-MRP4-his6 to perform Protein Expression Eukaryotic cells - P. pastoris human MRP4-his6

Get tips on using pSS2-GLURP- SERA5 to perform Protein Expression Prokaryotic cells - L. lactis SERA5 P. falciparum

Get tips on using pSS2-GLURP- MSPDBL2 to perform Protein Expression Prokaryotic cells - L. lactis MSPDBL2 P. falciparum

Get tips on using pUC19/Pr-EC to perform Protein Expression Eukaryotic cells - K. lactis α-l-Fucosidase