DNA isolation / purification Bacteria

A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. However, non-specific product amplification and primer-dimer formation during set-up are major causes of PCR failure. Nevertheless, high-quality DNA polymerase and optimize reaction buffers will certainly lead to a successful PCR reaction

A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. However, non-specific product amplification and primer-dimer formation during set-up are major causes of PCR failure. Nevertheless, high-quality DNA polymerase and optimize reaction buffers will certainly lead to a successful PCR reaction.

Get tips on using DNA Ligation Kit to perform DNA ligation

Get tips on using T4 DNA Ligase to perform DNA ligation

Get tips on using Fast DNA Ladder to perform DNA Ladder Fast

Get tips on using Supercoiled DNA Ladder to perform DNA Ladder Supercoiled

Get tips on using Supermix DNA Ladder to perform DNA Ladder 500 bp

Get tips on using 100bp DNA Ladder to perform DNA Ladder 100 bp

Get tips on using 1kb DNA Ladder to perform DNA Ladder 1 kb

Transfection is a powerful technique that enables the study of the function of genes and gene products in cells. Based on the nature of experiments, we may need a stable DNA transfection in cells for persistent gain-of-function or loss-of-function of the target gene. For stable transfection, integration of a DNA vector into the chromosome is crucial which requires selective screening and clonal isolation. By carefully selecting a viral delivery system and related reagents we can ensure safe and highly-efficient delivery of expression constructs for high-level constitutive or inducible expression in any mammalian cell type.