Protein Expression Prokaryotic cells A. cellulolyticus

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RNA isolation / purification Cells - primary porcine tracheal epithelial cells Experiment

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

RNA RNA isolation / purification Cells primary porcine tracheal epithelial cells
DNA isolation / purification Cells - Primary cells Bone marrow mononuclear cells Experiment

Isolating DNA from tissues and paraffin-embedded tissue samples can be challenging as double-stranded DNA is physically fragile and highly susceptible to exo- and endonucleases. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in the presence of DNAse inhibitors. Further, extracting DNA from the nucleus need specific methods by combining physical, mechanical and chemical lysis approaches,

DNA DNA isolation / purification Cells Primary cells Bone marrow mononuclear cells
DNA isolation / purification Cells - Primary cells Pseudomyxoma peritonei (PMP) cells Experiment

Isolating DNA from tissues and paraffin-embedded tissue samples can be challenging as double-stranded DNA is physically fragile and highly susceptible to exo- and endonucleases. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in the presence of DNAse inhibitors. Further, extracting DNA from the nucleus need specific methods by combining physical, mechanical and chemical lysis approaches,

DNA DNA isolation / purification Cells Primary cells Pseudomyxoma peritonei (PMP) cells
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Get tips on using RIPA Buffer to perform Protein isolation Mammalian cells - Mouse Epididymal fat

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Get tips on using RIPA Lysis Buffer, 10X to perform Protein isolation Mammalian cells - HaCaT

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Get tips on using RIPA Lysis Buffer System to perform Protein isolation Mammalian cells - HOG

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Get tips on using RIPA Lysis Buffer, 10X to perform Protein isolation Mammalian cells - STTG1

Products Merck Millipore RIPA Lysis Buffer, 10X

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