Protein Expression Prokaryotic cells A. tumefaciens

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When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

RNA RNA isolation / purification Cells primary mouse tracheal epithelial cells

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

RNA RNA isolation / purification Cells primary porcine tracheal epithelial cells

Isolating DNA from tissues and paraffin-embedded tissue samples can be challenging as double-stranded DNA is physically fragile and highly susceptible to exo- and endonucleases. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in the presence of DNAse inhibitors. Further, extracting DNA from the nucleus need specific methods by combining physical, mechanical and chemical lysis approaches,

DNA DNA isolation / purification Cells Primary cells Bone marrow mononuclear cells

Isolating DNA from tissues and paraffin-embedded tissue samples can be challenging as double-stranded DNA is physically fragile and highly susceptible to exo- and endonucleases. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in the presence of DNAse inhibitors. Further, extracting DNA from the nucleus need specific methods by combining physical, mechanical and chemical lysis approaches,

DNA DNA isolation / purification Cells Primary cells Pseudomyxoma peritonei (PMP) cells

Get tips on using RIPA Buffer to perform Protein isolation Mammalian cells - Human lung fibroblasts

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Get tips on using RIPA Buffer to perform Protein isolation Mammalian cells - Mouse Epididymal fat

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Get tips on using RIPA Buffer (10X) to perform Protein isolation Mammalian cells - Rat_Renal tissue

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Get tips on using TRI Reagent® to perform Protein isolation Mammalian cells - Mouse_Brown fat

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Get tips on using RIPA Lysis Buffer, 10X to perform Protein isolation Mammalian cells - HaCaT

Products Merck Millipore RIPA Lysis Buffer, 10X

Get tips on using RIPA Lysis Buffer System to perform Protein isolation Mammalian cells - HOG

Products Santa Cruz Biotechnology RIPA Lysis Buffer System

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