siRNA / miRNA gene silencing Mouse Embryonic stem cells

Get tips on using HyClone Minimal Essential Medium (MEM) variations: Liquid to perform Stem cell culture media Human salivary gland stem cells

Get tips on using PAXgene Tissue RNA/miRNA Kit to perform RNA isolation / purification Tissue - Rat Stomach

Get tips on using Dulbecco’s Modified Eagle’s Medium - high glucose to perform Stem cell culture media Mouse fibroblasts from meninges

Get tips on using EGMTM-2 Endothelial Cell Growth Medium-2 BulletKit to perform Stem cell culture media hLung Mesenchymal Stem Cells (MSCs)

Get tips on using PAXgene Tissue miRNA Kit to perform RNA isolation / purification Tissue - Rat Brain

Get tips on using PAXgene Tissue miRNA Kit to perform RNA isolation / purification Tissue - rat brain tissue

Get tips on using PAXgene Tissue miRNA Kit to perform RNA isolation / purification Tissue - rat spleen tissue

Get tips on using PAXgene Tissue miRNA Kit to perform RNA isolation / purification Tissue - rat heart muscle tissue

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

Get tips on using pSUPER.retro.puro to perform shRNA gene silencing Rat - MM1 SSH1