ChIP acH4 Human Bovine

Get tips on using EpiTect ChIP OneDay Kit to perform ChIP Mouse - MIN6

Get tips on using EpiTect ChIP qPCR Assays to perform ChIP Mouse - NIH3T3

Get tips on using EZ-Magna ChIP™ G - Chromatin Immunoprecipitation Kit to perform ChIP Human - Glioblastoma cell line

In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product in the experimental, add more DNA to the PCR reaction or increase the number of amplification cycles. Choose an alternate, ChIP-validated antibody if the antibody does not work.

Get tips on using EZ-Magna ChIP™ A/G Chromatin Immunoprecipitation Kit to perform ChIP Human - ASM

Get tips on using EZ-Magna ChIP™ A/G Chromatin Immunoprecipitation Kit to perform ChIP Human - AGS

Get tips on using EZ-Magna ChIP™ A/G Chromatin Immunoprecipitation Kit to perform ChIP Human - RCC

Get tips on using EZ-Magna ChIP™ A/G Chromatin Immunoprecipitation Kit to perform ChIP Human - OSCC

Get tips on using EZ-Magna ChIP™ A/G Chromatin Immunoprecipitation Kit to perform ChIP Human - PBMC

Get tips on using EZ-Magna ChIP™ A/G Chromatin Immunoprecipitation Kit to perform ChIP Human - Caco-2