crispr-human-repression-hpv-16-e7

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

Get tips on using "Illumina ™ TotalPrep ™ RNA Amplification Kit + Bio-16-UTP (10 mM) to perform Microarray RNA amplification & Labeling - Mouse cochlaea Biotin

Get tips on using lentiCRISPR v2 to perform CRISPR Mouse - Repression Mstn

Get tips on using lentiCRISPR v2 to perform CRISPR Mouse - Repression CCR-5

Get tips on using IFITM1 CRISPR Activation Plasmid (h) to perform CRISPR Human - Activation IFITM1

Get tips on using GRP 78 CRISPR Knockout and Activation to perform CRISPR Human - Activation GRP78

Get tips on using Multiplex CRISPR/Cas9 Assembly System Kit to perform CRISPR Human - Activation hATCB

Get tips on using GRP 78 CRISPR Knockout and Activation to perform CRISPR Human - Activation GRP 78

Get tips on using JAM-A CRISPR/Cas9 KO Plasmid (h) to perform CRISPR Human - Deletion F11R

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.