siRNA / miRNA gene silencing Rat RMC-1

Get tips on using HIF-1 alpha Antibody to perform Western blotting HIF-1 alpha

Get tips on using PE-Cy™7 Rat Anti-Mouse TNF to perform Flow cytometry Anti-bodies Mouse - TNF-α

Get tips on using Alexa Fluor® 488 Rat Anti-Mouse CD146 to perform Flow cytometry Anti-bodies Mouse - CD146/MCAM

Get tips on using SurePrint Human miRNA Microarrays to perform Microarray Human - Endometrial stromal cells miRNA-expression array (labelled)

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Get tips on using PE-Cy™7 Rat anti-Mouse CD117 to perform Flow cytometry Anti-bodies Mouse - CD117/c-kit

Get tips on using GDNF RECEPTOR ALPHA 1 to perform Immunohistochemistry Mouse - GFRA1

Get tips on using LAMP-1 Polyclonal Antibody to perform Autophagy assay cell type - RAW 264.7

Get tips on using pRSET A-FhFtn-1 to perform Protein Expression Prokaryotic cells - E. coli FhFtn-1

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.