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Cell cytotoxicity / Proliferation assay

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Get tips on using Atg5 (D5F5U) Rabbit mAb to perform Autophagy assay cell type - K562 cells

Products Cell Signaling Technology Atg5 (D5F5U) Rabbit mAb

Get tips on using SQSTM1 Antibody (D-3) to perform Autophagy assay cell type - K562 cells

Products Santa Cruz Biotechnology SQSTM1 Antibody (D-3)

Get tips on using ApopTag® Peroxidase In Situ Apoptosis Detection Kit to perform TUNEL assay cell type - FaDu human squamous cell carcinoma

Products Millipore ApopTag® Peroxidase In Situ Apoptosis Detection Kit

Human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) have been greatly used for studies on embryonic development and cell differentiation.iPSCs provide a stable source for either self-renewal or differentiation into suitable cells when cultured in a particular environment. Pluripotent cell culture was originally started by deriving cells from inner cell mass (ICM) from pre-implanted blastocysts, these were called embryonic stem cells. These cells after isolation can be grown on traditional extracellular matrices (like mouse embryonic fibroblasts, MEFs) or feeder-free culture systems. DMEM/F12 has been the most commonly used basal media in the culture of pluripotent cells. These cells are cultured at normal atmospheric oxygen levels, 21%, however, some studies have proposed that 4% oxygen tension may be better for hESC growth. Higher D-glucose concentration (4.2g/l) and osmolarity (320mOsm) that mimics the natural environment of embryonic tissue are optimal for the growth of hESCs. Supplements like N2 and/or B-27, in the presence of growth factors like bFGF, have been shown to increase pluripotency of these cells. bFGF, FGF2 and other ligands of receptor tyrosine kinases like IGF are also required or maintain self-renewal ability of these cells. TGF𝛃1, by its activation of SMAD2/3 signalling, also represses differentiation of iPSCs. Other compounds like ROCK inhibitors reduce blebbing and apoptosis in these cells to maintain their clonogenicity. However, an inhibitor for LIF (leukaemia inhibitory factor, which is one of the pluripotent genes) has an opposing effect. Therefore, it is important to understand the culture conditions and media composition that affect downstream signalling in hESCs or iPSCs that may lead to their differentiation.

Cell culture media Stem cell culture media Human bone mesenchymal stem cell (BMSC)

Get tips on using LIVE/DEAD™ Fixable Near-IR Dead Cell Stain Kit, for 633 or 635 nm excitation to perform Live / Dead assay mammalian cells - rat testicular tissue

Products Thermo Fisher Scientific LIVE/DEAD™ Fixable Near-IR Dead Cell Stain Kit, for 633 or 635 nm excitation

Get tips on using LC3B Antibody to perform Autophagy assay cell type - Rat cerebral coritcal tissue

Products Novus Biologicals LC3B Antibody
Anti-Atg5 Product

Get tips on using Anti-Atg5 to perform Autophagy assay cell type - Mouse white adipose tissue

Products MBL international corporation Anti-Atg5

Get tips on using Fluoro hROS to perform ROS assay cell type - MiaPaCa-2 pancreatic carcinoma

Products Cell Technology Fluoro hROS

Get tips on using BECN1 Antibody (H-300) to perform Autophagy assay cell type - INS-1E

Products Santa Cruz Biotechnology BECN1 Antibody (H-300)

Get tips on using Autophagy Inhibitor, 3-MA to perform Autophagy assay cell type - INS-1E

Products Sigma-Aldrich Autophagy Inhibitor, 3-MA

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