ChIP acH4 Mouse Canine

Get tips on using VWF Antibody (C-12): sc-365712 to perform Immunohistochemistry Mouse - vWF

Get tips on using VEGF Antibody (C-1): sc-7269 to perform Immunohistochemistry Mouse - VEGFA

Get tips on using Anti-Peptide YY/PYY antibody (ab131246) to perform Immunohistochemistry Mouse - PYY

Get tips on using Anti-Glucagon antibody produced in rabbit to perform Immunohistochemistry Mouse - Glucagon

Get tips on using Phospho-Histone H3 (Ser10) Antibody #9701 to perform Immunohistochemistry Mouse - PHH3

Get tips on using Active BDNF (Human, Rat) ELISA Kit to perform ELISA Mouse - GDNF

Get tips on using REDExtract-N-Amp™ PCR ReadyMix™ to perform PCR Mouse

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary mouse cortical neurons

Get tips on using Timp2 siRNA to perform siRNA / miRNA gene silencing Mouse - BV2 TIMP-2