Site Directed Mutagenesis (SDM) Mouse Deletion

A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. A quantitative, real-time PCR reaction typically includes all of that plus a probe that can be detected fluorescently as the reaction runs, with no gel required. for detection. However, non-specific product amplification and primer-dimer formation during set-up are major causes of PCR failure. Nevertheless, high-quality DNA polymerase and optimize reaction buffers will certainly lead to a successful PCR reaction.

A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. A quantitative, real-time PCR reaction typically includes all of that plus a probe that can be detected fluorescently as the reaction runs, with no gel required. for detection. However, non-specific product amplification and primer-dimer formation during set-up are major causes of PCR failure. Nevertheless, high-quality DNA polymerase and optimize reaction buffers will certainly lead to a successful PCR reaction.

Get tips on using Smooth Muscle Actin Antibody (B4): sc-53142 to perform Immunohistochemistry Mouse - SMA

Get tips on using Anti-alpha smooth muscle Actin antibody (ab5694) to perform Immunohistochemistry Mouse - SMA

Get tips on using Silencer® siRNA(m) Nostrin to perform siRNA / miRNA gene silencing Mouse - MS1 Nostrin

Get tips on using Silencer®_Skiv2l2 siRNA (m) to perform siRNA / miRNA gene silencing Mouse - P19 Skiv2l2

Get tips on using Muse® Cell Cycle Assay Kit to perform Cell cycle assay mouse - C2C12

Get tips on using Muse® Cell Cycle Assay Kit to perform Cell cycle assay mouse - L929

Get tips on using Gibco™ DMEM, high glucose to perform Stem cell culture media Mouse pericytes

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Tissue - Mouse Small intestine