DNA transfection Mammalian cells Primary cells

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Get tips on using RIPA Buffer to perform Protein isolation Mammalian cells - Rat_Circumvallate papillae

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Get tips on using Nuclear Extract Kit to perform Protein isolation Mammalian cells - HOG

Get tips on using RIPA Buffer (10X) to perform Protein isolation Mammalian cells - Huh7

Get tips on using Complete Kit for Human Whole Blood CD34+ Cells to perform Cell Isolation CD34+ cells

Get tips on using pCEP4 Mammalian Expression Vector to perform Protein expression and purification Bacteria - Origami™ B (DE3) ABCA4