Protein expression and purification Mammalian cells HeLa

Get tips on using pTRAkc-rbcs1-cTP/pRIC 3.0 to perform Protein Expression Prokaryotic cells - A. tumefaciens BFDV cp

Get tips on using pIEX-4-Crypα-6His-mMBP-UMODpXR to perform Protein Expression Eukaryotic cells - S. frugiperda mMBP

Get tips on using Pierce™ Coomassie Plus (Bradford) Assay Reagent to perform Protein quantification Mammalian cells - OUMS-27

DNA isolation and purification is the first critical step in sample preparation that helps ensure optimal performance of downstream assays like PCR, microarrays, and sequencing. Failure in yielding high-quality DNA would be the major reason that DNA doesn't work for the downstream application. To circumvent this, one should follow the recommended storage conditions to minimize DNA degradation by nucleases and shouldn't overload the purification system.

Get tips on using pST44-yAda3Δ2HIS-yAda2Δ1-yGcn5 to perform Protein Expression Prokaryotic cells - E. coli Ada2/Ada3/Gcn5 complex

A gross majority of classical apoptotic attributes can be quantitatively examined by flow cytometry, the preferred platform for rapid assessment of multiple cellular attributes at a single-cell level. However, sample preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

DNA microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.

Get tips on using Guava Nexin Annexin V Assay to perform Apoptosis assay cell type - HeLa cells

Get tips on using Viability/Cytotoxicity Assay kit for Bacteria Live and Dead Cells to perform Live / Dead assay bacteria - Salmonella enterica

Get tips on using pPICZαB/α-amylase to perform Protein Expression Eukaryotic cells - P. pastoris G. stearothermophilus SR74 α-amylase