CRISPR Mouse Deletion ES (embryonic stem) cells

Get tips on using lenti MS2-P65-HSF1_Hygro to perform CRISPR Human - Activation PDIA1

Get tips on using lenti MS2-P65-HSF1_Hygro to perform CRISPR Human - Activation APOBEC3G

Get tips on using pcDNA-dCas9-p300 Core to perform CRISPR Human - Activation MASPIN

Get tips on using pAC2-dual-dCas9VP48-sgExpression to perform CRISPR Human - Activation IL1RN

Get tips on using UltraCruz® Transfection Reagent to perform CRISPR Human - Activation GRP78

Get tips on using pAC2-dual-dCas9VP48-sgExpression to perform CRISPR Human - Activation SOX2

Isolating RNA from tissues and paraffin-embedded tissue samples can be challenging due to cross-linking of biomolecules and fragmented nucleic acids. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in presence of RNAse inhibitors. The homogenization process should be carried out on dry ice to maintain the integrity of RNA.

Get tips on using Gibco™IMDM to perform Stem cell Differentiation media hiPSCs or hESCs differentiation to Embryoid body (EB)

Get tips on using Monoclonal Mouse Anti-Human Cytokeratin 7 (Dako Omnis) Clone OV-TL 12/30 to perform Immunohistochemistry Human - CK7

Get tips on using DIA-310: Anti-CD31 (Ms) from Rat (Clone: SZ31) for mouse FFPE tissue to perform Immunohistochemistry CD31 - Rabbit Rat -NA-