Protein expression and purification Insect cells Hi5

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Isolating RNA from tissues and paraffin-embedded tissue samples can be challenging due to cross-linking of biomolecules and fragmented nucleic acids. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in presence of RNAse inhibitors. The homogenization process should be carried out on dry ice to maintain the integrity of RNA.

RNA RNA isolation / purification Tissue Mouse Small intestine

What is the difference between endothelial cells and HUVEC cells for angiogenesis assay?

Discussions endothelial cells vs HUVEC cells?

Get tips on using QIAexpress Type IV Kit to perform Protein tag Purification of His-tagged proteins

Products Qiagen QIAexpress Type IV Kit

Get tips on using UNveil Unstained Protein Ladder(10 to 200 kDa) to perform Protein Ladder Unstained

Products BIO-HELIX UNveil Unstained Protein Ladder(10 to 200 kDa)

Get tips on using Unstained Protein Standard, Broad Range (10-200 kDa) to perform Protein Ladder Unstained

Products New England BioLabs Unstained Protein Standard, Broad Range (10-200 kDa)

Get tips on using Odyssey® One-Color Protein Molecular Weight Marker to perform Protein Ladder Immunofluorescence

Products LI-COR Odyssey® One-Color Protein Molecular Weight Marker

Get tips on using BLUEstain™ 2 Protein ladder, 5-245 kDa to perform Protein Ladder Prestained

Products GOLDBIO BLUEstain™ 2 Protein ladder, 5-245 kDa

Get tips on using IRIS9 Prestained Protein Ladder(15 to 180 kDa) to perform Protein Ladder Prestained

Products BIO-HELIX IRIS9 Prestained Protein Ladder(15 to 180 kDa)

Proteins Protein tag Detection of biotinylated proteins

Get tips on using Color-coded Prestained Protein Marker, High Range (43-315 kDa) #12949 to perform Protein Ladder Prestained

Products Cell Signaling Technology Color-coded Prestained Protein Marker, High Range (43-315 kDa) #12949

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