Microarray Gene expression arrays Mouse

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.

Get tips on using Mouse TIM-1/KIM-1/HAVCR Quantikine ELISA Kit to perform ELISA Mouse - KIM-1

Get tips on using Mouse/Rat IGF-I/IGF-1 Quantikine ELISA Kit to perform ELISA Mouse - IGF-I

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Get tips on using Cnp siRNA to perform siRNA / miRNA gene silencing Mouse - BV2 CNPase

Get tips on using Wwtr1 siRNA to perform siRNA / miRNA gene silencing Mouse - C2C12 Taz

Get tips on using Bambi siRNA to perform siRNA / miRNA gene silencing Mouse - C2C12 BAMBI

Get tips on using Stk11 siRNA to perform siRNA / miRNA gene silencing Mouse - C2C12 Stk11

Get tips on using Cxcr4 siRNA to perform siRNA / miRNA gene silencing Mouse - C2C12 CXCR4

Get tips on using Dusp3 siRNA to perform siRNA / miRNA gene silencing Mouse - BMDMs Dusp3