siRNA / miRNA gene silencing Mouse CT26

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.

Get tips on using Monoclonal Mouse Anti-Bromodeoxyuridine (Concentrate) Clone Bu20a to perform Immunohistochemistry Mouse - BrdU

Get tips on using Mouse OPN / Osteopontin PicoKine™ ELISA Kit to perform ELISA Mouse - OPN

Get tips on using Mouse VEGF PicoKine™ Fast ELISA Kit to perform ELISA Mouse - VEGF

Get tips on using Mouse/Rat Osteopontin (OPN) Quantikine ELISA Kit to perform ELISA Mouse - OPN

Get tips on using Mouse Total MMP-9 Quantikine ELISA Kit to perform ELISA Mouse - MMP9

Get tips on using GFAP (GA5) Mouse mAb #3670 to perform Immunohistochemistry Rat - GFAP

Get tips on using Human/Mouse NLRP3/NALP3 Antibody to perform Western blotting NLRP3