Cell cycle can be challenging due to difference introduced by sample handling, timing, and difference within the sample. Downstream instriuments to analyse cell cycle (Multicolor flow cytometry and multicolor imaging) can answer these challenges. Relevant markers can be combined with cell phenotyping markers to look at events within subpopulations of cells.
When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.
As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.
As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.
Get tips on using CultreCoat Low BME Cell Invasion Assay, 96 well to perform Cell migration / Invasion cell type - HN4
Get tips on using CultreCoat Low BME Cell Invasion Assay, 96 well to perform Cell migration / Invasion cell type - SCC2
Get tips on using CultreCoat Low BME Cell Invasion Assay, 96 well to perform Cell migration / Invasion cell type - SCC104
Get tips on using CultreCoat Low BME Cell Invasion Assay, 96 well to perform Cell migration / Invasion cell type - SCC6
Get tips on using CultreCoat Low BME Cell Invasion Assay, 96 well to perform Cell migration / Invasion cell type - SCC4
Get tips on using CultreCoat BME Cell Invasion Optimization Assay, 96 well to perform Cell migration / Invasion cell type - H1299
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