Mammalian cell culture media

Get tips on using STEMdiff™ Definitive Endoderm Kit to perform Stem cell Differentiation media Differentiation of Human hESCs into Endoderm

Get tips on using Gibco™DMEM, low glucose, pyruvate to perform Stem cell Differentiation media Glioma differentiation Human astrocytic lineage

Get tips on using Gibco™DMEM, high glucose, pyruvate to perform Stem cell Differentiation media Glioma differentiation Human astrocytic lineage

Get tips on using Gibco™Ham's F-12 Nutrient Mix to perform Stem cell Differentiation media hESCs differentiation into SFEBq

Get tips on using Gibco™IMDM, powder to perform Stem cell Differentiation media hPSCs or iPSCs differentiation into Lung progenitor cells

Get tips on using Gibco™ MEM α, Nucleosides to perform Stem cell Differentiation media Human oogonial stem cells differentiation into oocytes

Get tips on using Gibco™DMEM/F-12 to perform Stem cell Differentiation media iPSCs or hESCs differentiation into Neuronal cells

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Get tips on using Gibco™StemPro™ Chondrogenesis Differentiation Kit to perform Stem cell Differentiation media hMSCs differentiation into Chondrogenic cells

Get tips on using Gibco™StemPro™ Osteogenesis Differentiation Kit to perform Stem cell Differentiation media hMSCs differentiation into osteogenic cells