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Get tips on using Wizard® Genomic DNA Purification Kit to perform DNA isolation / purification Cells - Primary cells Rat astrocytes

Hello! I used Trizol to extract total RNA from in-vitro cultured bacteria (1 X 10^8 cells). After phase separation, I mixed ~0.4 ml of the upper phase which contains RNA with 0.5 mL cold isopropanol. However, the amount of RNA when measured in Nanodrop was very low. In addition, the ratio between 260 and 230 was around 0.1 to 0.5. Is there a chance that my sample was contaminated by the Trizol reagent? When I collected the aqueous phase I made sure to not touch the lower phase. What should I do?

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Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary rat dermal fibroblasts

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Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary rat cardiac fibroblasts

Get tips on using Magnetic mRNA Isolation Kit to perform RNA isolation / purification Tissue - Rat Pineal gland