siRNA / RNAi /miRNA transfection Mouse Glomerular Mesangial cells

Get tips on using Mouse BDNF PicoKine™ ELISA Kit to perform ELISA Mouse - BDNF

Get tips on using IntestiCult™ Organoid Growth Medium (Mouse) to perform 3D Cell Culture Media Mouse gastric cancer organoids

Get tips on using IntestiCult™ Organoid Growth Medium (Mouse) to perform 3D Cell Culture Media Mouse small intestinal organoids

Get tips on using Mouse TNF alpha ELISA Kit (ab208348) to perform ELISA Mouse - TNF-alpha

Get tips on using Mouse TNF-alpha Quantikine ELISA Kit to perform ELISA Mouse - TNF-alpha

Get tips on using Mouse RANKL ELISA Kit (TNFSF11) (ab100749) to perform ELISA Mouse - RANK L

Get tips on using Mouse Lipocalin-2/NGAL DuoSet ELISA to perform ELISA Mouse - NGAL/LCN2

Get tips on using Mouse Dkk-1 Quantikine ELISA Kit to perform ELISA Mouse - Dkk-1

Get tips on using Mouse ICAM1 ELISA Kit (CD54) (ab100688) to perform ELISA Mouse - ICAM-1/CD54

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.