ChIP acH3 Canine Rat

Get tips on using Pan Ras Monoclonal Antibody (Ras10) to perform Western blotting Ras

Get tips on using Donkey anti-rabbit IgG to perform Immunohistochemistry Anti-rabbit IgG - Donkey Rabbit Rhodamin red

Get tips on using Connexin 43 Antibody #3512 to perform Western blotting CX43

Get tips on using Connexin 43 Polyclonal Antibody to perform Western blotting CX43

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.