Immunohistochemistry Anti-mouse IgG Donkey Mouse

Get tips on using Anti-PI 3 Kinase p85 alpha antibody [EP380Y] to perform Autophagy assay cell type - HepG2

Get tips on using anti-p62 Protein, C-Terminal Specific Polyclonal Antibody to perform Autophagy assay cell type - MDA-MB-231

Get tips on using Anti-ATG5 (C-terminal) antibody produced in rabbit to perform Autophagy assay cell type - Rat spinal cord tissue

Get tips on using SCGB1A1 antibody (Secretoglobin, Family 1A, Member 1 (Uteroglobin)) (Middle Region) to perform Immunohistochemistry Human - SCGB1A1 /CC10

Get tips on using Recombinant Anti-STAT3 (phospho S727) antibody [E121-31] (ab32143) to perform Western blotting STAT3

Get tips on using Recombinant Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (ab181537) to perform Western blotting Ubiquitin

Get tips on using CD171 (L1CAM) Antibody, anti-human, PE-Vio® 770, REAfinity™ to perform Flow cytometry Anti-bodies Human - CD171/L1CAM

Get tips on using Recombinant Anti-IKK alpha + IKK beta antibody [EPR16628] (ab178870) to perform Western blotting IKK Alpha

Get tips on using Recombinant Anti-Cyclin D1 antibody [EPR2241] - C-terminal (ab134175) to perform Western blotting Cyclin D1

Flow cytometry is an immunophenotyping technique whereby sing cell suspensions are stained for either cell surface markers or intracellular proteins by fluorescently-labelled antibodies and analyzed with a flow cytometer, where fluorescently-labelled molecules are excited by the laser to emit light at varying wavelengths, which is then detected by the instrument. There are several key criteria which are required to be kept in mind while designing a flow experiment- 1. Antibody titration (optimal dilution of antibodies should be calculated in order to avoid over- or under- saturated signals for proper detection of surface and intracellular markers), 2. Precision (3 or more replicates of the sample should be used per experiment), 3. Specificity (proper isotype controls should be included in the experiment), 4. Day-to-day variability (experiments should be repeated 3 or more times to ensure consistency and avoid variability due to flow cytometer settings), 5. Antibody interaction (Fluorescence minus one or FMO should be used, which is the comparison of signals from panel minus one antibody vs. the full panel), and 6. Antibody stability (fluorescently-labelled antibodies should be stored at 4C).