siRNA / miRNA gene silencing Rat Brain endothelial cells

Get tips on using Purified Rat Anti-Mouse CD117 to perform Flow cytometry Anti-bodies Mouse - CD117/c-kit

Get tips on using PE Rat Anti-Mouse CD138 to perform Flow cytometry Anti-bodies Mouse - CD138/Syndecan-1

Get tips on using PE Rat Anti-Mouse CD140A to perform Flow cytometry Anti-bodies Mouse - CD140/PDGFR-α

Get tips on using Biotin Rat Anti-Mouse CD106 to perform Flow cytometry Anti-bodies Mouse - CD106/Vcam-1

Get tips on using Purified Rat Anti-Mouse CD106 to perform Flow cytometry Anti-bodies Mouse - CD106/Vcam-1

Get tips on using Rat Retinol binding protein 4,RBP-4 ELISA Kit to perform ELISA Rat - RBP4

Get tips on using CellROX™ Deep Red Reagent, for oxidative stress detection to perform ROS assay cell type - human umbelical vein endothelial cells (HUVEC)

Get tips on using Human/Mouse/Rat Activin A Quantikine ELISA Kit to perform ELISA Rat - Activin

Get tips on using Cellular Senescence Flow Cytometry Assay to perform Reporter gene assay β-galactosidase substrates - rat mesenchymal stem cells (MSCs)

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.