Mammalian cell culture media

Get tips on using GenJet™ In Vitro DNA Transfection Reagent to perform DNA transfection Mammalian cells - Immortalized cell lines MCF-7

Get tips on using X-tremeGENE™ HP DNA Transfection Reagent to perform DNA transfection Mammalian cells - Immortalized cell lines PANC-1

Get tips on using Gibco™DMEM/F-12 to perform Stem cell Differentiation media mPSCs/mESCs differentiation to Embryoid body (EB)

Get tips on using Gibco™Glasgow's MEM (GMEM) to perform Stem cell Differentiation media mPSCs/mESCs differentiation to Embryoid body (EB)

Get tips on using Gibco™KnockOut™ DMEM to perform Stem cell Differentiation media mPSCs/mESCs differentiation to Embryoid body (EB)

Get tips on using STEMdiff™ Pancreatic Progenitor Kit to perform Stem cell Differentiation media Differentiation of Human hESCs into pancreatic progenitors

Get tips on using Gibco™KnockOut™ Serum Replacement to perform Stem cell Differentiation media hESCs differentiation into cortical neuroepithelium (NE)

Get tips on using Gibco™ PSC Cardiomyocyte Differentiation Kit to perform Stem cell Differentiation media hESCs or hiPSCs differentiation into Cardiomyocytes

Get tips on using STEMdiff™ Hematopoietic Kit to perform Stem cell Differentiation media hiPSCs differentiation into CD43+ primitive hematopoietic progenitor cells (HPCs)

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.