DNA isolation and purification is the first critical step in sample preparation that helps ensure optimal performance of downstream assays like PCR, microarrays, and sequencing. Failure in yielding high-quality DNA would be the major reason that DNA doesn't work for the downstream application. To circumvent this, one should follow the recommended storage conditions to minimize DNA degradation by nucleases and shouldn't overload the purification system.
Get tips on using miRcute miRNA Isolation Kit to perform RNA isolation / purification Cells - immortalized H1299
Get tips on using EpiQuik Chromatin Immunoprecipitation (ChIP) Kit to perform ChIP Rat - INS-1
Transfection is a powerful technique that enables the study of the function of genes and gene products in cells. Based on the nature of experiments, we may need a stable DNA transfection in cells for persistent gain-of-function or loss-of-function of the target gene. For stable transfection, integration of a DNA vector into the chromosome is crucial which requires selective screening and clonal isolation. By carefully selecting a viral delivery system and related reagents we can ensure safe and highly-efficient delivery of expression constructs for high-level constitutive or inducible expression in any mammalian cell type.
Get tips on using 1 kb DNA ladder-TLAD-1K to perform DNA Ladder 1 kb
Get tips on using ZO-1 Monoclonal Antibody (ZO1-1A12) to perform Western blotting ZO-1
Get tips on using Absolutely RNA miRNA Kit to perform RNA isolation / purification Cells - immortalized HT-29
Get tips on using BIO-MPM-1 SFM to perform Mammalian cell culture media HC
Get tips on using APG5 Antibody (C-1) to perform Autophagy assay cell type - A549
Get tips on using TriDye™ 1 kb DNA Ladder to perform DNA Ladder 1 kb
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