Get tips on using EpiTect ChIP OneDay Kit to perform ChIP Human - HUH-7
Get tips on using EpiTect ChIP OneDay Kit to perform ChIP Human - MIA PaCa-2
In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product in the experimental, add more DNA to the PCR reaction or increase the number of amplification cycles. Choose an alternate, ChIP-validated antibody if the antibody does not work.
Get tips on using ChIPAb+ Trimethyl-Histone H3 (Lys36) - ChIP Validated Antibody and Primer Set to perform ChIP Anti-bodies H3K36me3
Get tips on using Chromatin Immunoprecipitation (ChIP) Assay Kit to perform ChIP Rat - Megakaryocytes
Get tips on using EpiQuik Chromatin Immunoprecipitation (ChIP) Kit to perform ChIP Rat - Colon
Get tips on using Chromatin Immunoprecipitation (ChIP) Assay Kit to perform ChIP Rat - NRK52E
Get tips on using Pierce™ Agarose ChIP Kit to perform ChIP Rat - NRK52E
Get tips on using Chromatin Immunoprecipitation (ChIP) Assay Kit to perform ChIP Rat - Liver
Get tips on using Pierce™ Magnetic ChIP Kit to perform ChIP Rat - Pancreas
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