Western blot 1,4 β-N-acetyl-D-glucosamine

Get tips on using VEGF-D siRNA (h) to perform siRNA / miRNA gene silencing Human - Caki-2 VEGF-D

Get tips on using pEV-UDP-D to perform Protein Expression Prokaryotic cells - E. coli dehydrogenase

Get tips on using pHT43-BMP2-D to perform Protein Expression Prokaryotic cells - B. subtilis rhBMP2

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.

Get tips on using 2-D Quant Kit to perform Protein quantification Colorimetric method

Get tips on using 2-D Quant Kit to perform Protein quantification Mammalian cells - SiHa

Get tips on using SQSTM1 Antibody (D-3) to perform Autophagy assay cell type - A549

Get tips on using SQSTM1 Antibody (D-3) to perform Autophagy assay cell type - HepG2

Get tips on using SQSTM1 Antibody (D-3) to perform Autophagy assay cell type - Goblet cells