dna-quantification-human-hela

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Get tips on using Investigator Quantiplex HYres Kit to perform DNA quantification Blood

Products Qiagen Investigator Quantiplex HYres Kit

Get tips on using ON-TARGETplus Human PLK1 (5347) siRNA - Individual to perform siRNA / miRNA gene silencing Human - HeLa PLK-1

Products Dharmacon ON-TARGETplus Human PLK1 (5347) siRNA - Individual

Get tips on using Accell Human VDAC1 (7416) siRNA - Set of 4 to perform siRNA / miRNA gene silencing Human - HeLa VDAC1

Products Dharmacon Accell Human VDAC1 (7416) siRNA - Set of 4

Get tips on using QuantiFluor® dsDNA System to perform DNA quantification Mouse - EMT6

Products Promega QuantiFluor® dsDNA System

Transfection is a powerful technique that enables the study of the function of genes and gene products in cells. Based on the nature of experiments, we may need a stable DNA transfection in cells for persistent gain-of-function or loss-of-function of the target gene. For stable transfection, integration of a DNA vector into the chromosome is crucial which requires selective screening and clonal isolation. By carefully selecting a viral delivery system and related reagents we can ensure safe and highly-efficient delivery of expression constructs for high-level constitutive or inducible expression in any mammalian cell type.

DNA DNA transfection Mammalian cells Primary cells Human lung fibroblasts (HLF)

Get tips on using CometAssay Electrophoresis System II to perform DNA Damage Assay HeLa

Products Bio-Techne CometAssay Electrophoresis System II

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

DNA DNA methylation profiling Whole genome profiling MCF-7, MDA-MB-453 human breast cancer

Get tips on using QuantiFluor® dsDNA System to perform DNA quantification Mouse - NIH 3T3

Products Promega QuantiFluor® dsDNA System

Get tips on using Qubit dsDNA HS Assay Kit to perform DNA quantification Brain tissue

Products Thermo Fisher Scientific Qubit dsDNA HS Assay Kit

Transfection is a powerful technique that enables the study of the function of genes and gene products in cells. Based on the nature of experiments, we may need a stable DNA transfection in cells for persistent gain-of-function or loss-of-function of the target gene. For stable transfection, integration of a DNA vector into the chromosome is crucial which requires selective screening and clonal isolation. By carefully selecting a viral delivery system and related reagents we can ensure safe and highly-efficient delivery of expression constructs for high-level constitutive or inducible expression in any mammalian cell type.

DNA DNA transfection Mammalian cells Primary cells Human prenatal cardiac progenator cells

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