Live / Dead assay

Isolating RNA from tissues and paraffin-embedded tissue samples can be challenging due to cross-linking of biomolecules and fragmented nucleic acids. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in presence of RNAse inhibitors. The homogenization process should be carried out on dry ice to maintain the integrity of RNA

Get tips on using Fluoro H202 to perform ROS assay cell type - Capan-2, PANC-1 pancreatic carcinoma

Get tips on using psiCHECK-2vector to perform Reporter gene assay luciferase - HEK 293 human embryonic kidney cells

Get tips on using pGL3-Basic Vector to perform Reporter gene assay luciferase - vascular smooth muscle cells (VSMC)

Get tips on using CometAssay Electrophoresis System II to perform DNA Damage Assay Human Skin Fibroblast Cell (FSK)

Get tips on using CometChip Electrophoresis Starter Kit to perform DNA Damage Assay Human bronchial epithelial cells (hBE)

Get tips on using LAMP-1 Antibody (H4A3) to perform Autophagy assay cell type - Proximal tubular cells (rPT)

Get tips on using Atg7 (D12B11) Rabbit mAb to perform Autophagy assay cell type - Hippocampal neural stem cells

Get tips on using LC3B monoclonal antibody (2G6) to perform Autophagy assay cell type - MEFs (mouse embryonic fibroblasts)