Angiogenesis assay mouse

Get tips on using DeadEnd™ Colorimetric TUNEL System to perform TUNEL assay cell type - Mouse skeletal muscle cells

Get tips on using Anti-LC3B antibody produced in rabbit to perform Autophagy assay cell type - Mouse lung tissue

Get tips on using Anti-LC3B antibody produced in rabbit to perform Autophagy assay cell type - Mouse embryonic fibroblasts

Get tips on using CYTO-ID® Autophagy detection kit to perform Autophagy assay cell type - Mouse embryonic fibroblasts

Get tips on using Live/Dead cell Staining Kit II to perform Live / Dead assay mammalian cells - mouse iPSC

Get tips on using Zombie Violet™ Fixable Viability Kit to perform Live / Dead assay mammalian cells - mouse microglia

Get tips on using Zombie UV™ Fixable Viability Kit to perform Live / Dead assay mammalian cells - mouse splenocytes

Get tips on using TiterTACS™ Colorimetric Apoptosis Detection Kit to perform TUNEL assay cell type - Mouse endothelial cells

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.