siRNA / miRNA gene silencing Human SHSY5Y

Get tips on using HO-1 (human), ELISA kit to perform ELISA Human - HO-1

Get tips on using GM-CSF Human ELISA Kit to perform ELISA Human - GM-CSF

Get tips on using Human GM-CSF DuoSet ELISA to perform ELISA Human - GM-CSF

Get tips on using Cytochrome C Human ELISA Kit to perform ELISA Human - Cytochrome C

Get tips on using Human BMP2 ELISA Kit (ab119581) to perform ELISA Human - BMP-2

DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.

Get tips on using Purified Mouse Anti-Human CD135 to perform Flow cytometry Anti-bodies Human - CD135

Get tips on using PE Mouse Anti-Human CD135 to perform Flow cytometry Anti-bodies Human - CD135

Get tips on using PE Mouse Anti-Human CD122 to perform Flow cytometry Anti-bodies Human - CD122

Get tips on using PE Mouse Anti-Human CD116 to perform Flow cytometry Anti-bodies Human - CD116