reporter-gene-assay-luciferase-bhk-21-baby-hamster-kidney-cells

Get tips on using OxiSelect™ Intracellular ROS Assay Kit (Green Fluorescence) to perform ROS assay cell type - MiaPaCa-2 pancreatic carcinoma

Get tips on using OxiSelect™ Intracellular ROS Assay Kit (Green Fluorescence) to perform ROS assay cell type - SH-SY5Y human neuroblastoma

Get tips on using Cultrex® BME Cell Invasion Assay to perform Cell migration / Invasion cell type - RAW 264.7

Get tips on using Cultrex® BME Cell Invasion Assay to perform Cell migration / Invasion cell type - PC-3

Get tips on using Cultrex® BME Cell Invasion Assay to perform Cell migration / Invasion cell type - PANC-1

Get tips on using AMPIPROBE® Candida 1 Assay Kit to perform Cell Culture Contamination Detection Kit Yeast

Get tips on using QCM™ Gelatin Invadopodia Assay (Green) to perform Cell migration / Invasion cell type - HT-1080

Get tips on using QCM™ Gelatin Invadopodia Assay (Green) to perform Cell migration / Invasion cell type - HT-1080

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.