Immunohistochemistry 53BP2 phospho (ser-25) Rabbit IgG Human

Get tips on using Human Ubiquitin/Ubiquitin+1 Antibody to perform Western blotting Ubiquitin

Get tips on using Human BDNF ELISA Kit (ab212166) to perform ELISA Mouse - BDNF

Get tips on using Human/Mouse BDNF DuoSet ELISA to perform ELISA Mouse - BDNF

Get tips on using Human SCF ELISA Kit (ab100636) to perform ELISA Rat - SC

Short hairpin or small hairpin RNA (shRNA) is artificial RNA, which has a hairpin loop structure, and uses inherent microRNA (miRNA) machinery to silence target gene expression. This is called RNA interference (RNAi). These can be delivered via plasmids or viral/bacterial vectors. Challenges in shRNA-mediated gene silencing include: 1. Off-target silencing, 2. Packaging shRNA encoding lentivirus, and 3. Stable transduction in cells. RNAi have been designed to have anywhere from 19-27 bs, but the most effective design has 19 bp. In case commercial shRNAs are not available, potential target sites can be chosen within exon, 5’- or 3’ UTR, depending on which splice variants of the gene are desired. One should use the latest algorithms and choose at least two different sequences, targeting different regions, in order to have confidence in overcoming off-target effects. A BLAST search after selecting potential design will eliminate potential off-target sequences. For the second challenge, sequencing the vector using primers for either strand (50-100 bp upstream) is suggested, along with using enzymatic digestion on agarose gel for the vector. Next, once the shRNA-containing vector is packaged in a virus, it is important to check the viral titer before transduction. Finally, using a marker in the lentiviral vector (fluorescent protein or antibiotic resistance), along with qPCR for target gene expression can help in determining efficacy of transduction and shRNA on its target site.

Get tips on using GeneChip® Human Genome U133 Plus 2.0 Array to perform Microarray Human - Endometrial stromal cells Expression array

Get tips on using Human VWF / von Willebrand Factor ELISA Kit to perform ELISA Human - VWF-A2

Get tips on using Human TNF Alpha PicoKine™ ELISA Kit to perform ELISA Human - TNF-alpha

Get tips on using Human Lipocalin-2 ELISA Kit (NGAL) (ab119600) to perform ELISA Human - NGAL/LCN2

Get tips on using Human Lipocalin-2/NGAL Quantikine ELISA Kit to perform ELISA Human - NGAL/LCN2