Wound healing assay cell type human

Western blotting is a widely used technique to size separate proteins from a pool of cell or tissue lysates. The technique has 4 major steps: a) gel electrophoresis, b) blocking and treatment with antigen specific antibody, c) treatment with secondary antibody and finally d) detection and visualization. Though western blotting is a widely used technique, detection of specific proteins depends on several factors, the major ones are antibody concentration, incubation time and washing steps. Key points for obtaining clean blots are: always prepare fresh buffer solutions and optimize antibody concentration. Given the advent of high-throughput protein analysis and a push to limit the use of lab consumables, onestep antibodies are developed which recognise protein of interest and also contain a detection label.

Get tips on using Image-IT™ LIVE Green Reactive Oxygen Species Detection Kit, for microscopy to perform ROS assay cell type - H9c2 rat cardiomyocytes

Get tips on using LC3A/B (D3U4C) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) #13082 to perform Autophagy assay cell type - RAW 264.7

Get tips on using EpiQuik Dnmt3A Assay Kit to perform DNA methylation profiling Whole genome profiling - human peripheral blood mononuclear cells

Get tips on using Qubit RNA HS Assay Kit to perform RNA quantification Fuorimetric - human trophoblast cells

Get tips on using BD Cycletest™ Plus DNA Kit to perform Cell cycle assay human - A2780

Get tips on using FxCycle™ PI/RNase Staining Solution to perform Cell cycle assay human - SW480

Get tips on using FxCycle™ PI/RNase Staining Solution to perform Cell cycle assay human - U266

Get tips on using BD Cycletest™ Plus DNA Kit to perform Cell cycle assay human - K562

DNA damage assay is a standard method for determining in-vivo/in-vitro genotoxicity by measuring the breaks in the DNA chain of animal and plant cells. Initial DNA damage leads to cell cycle arrest and, at the final stages, leads to induction of senescence or cell death (apoptosis, necrosis, autophagy, or mitotic catastrophe). Detection of DNA damage from mild to moderate to severe is challenging when studying genotoxicity in the pool of cells. It is favorable to use DNA damage assay kits available for prominent identification of the extent of damage in the analysis.