shRNA gene silencing Human Islets of langerhans Negative control (scrambled)

Get tips on using SignalSilence® NF-κB p65 siRNA I #6261 to perform siRNA / miRNA gene silencing Rat - H9c2 NF-κB RelA (p65)

DNA microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.

DNA microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Get tips on using Wizard® Genomic DNA Purification Kit to perform DNA isolation / purification Bacteria - Gram negative Haemophilus influenzae

Get tips on using Wizard® Genomic DNA Purification Kit to perform DNA isolation / purification Bacteria - Gram negative Salmonella enterica

Get tips on using Wizard® Genomic DNA Purification Kit to perform DNA isolation / purification Bacteria - Gram negative Helicobacter pylori

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Bacteria - Gram negative Vibro cholerae

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Bacteria - Gram negative Legionella pneumophilia

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Bacteria - Gram negative Borrelia burgdorferi