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Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Cells - immortalized U87

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Cells - immortalized T47D

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Cells - immortalized SiHa

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Cells - immortalized A549

Get tips on using QuantiFluor® dsDNA System to perform DNA quantification MCF10A breast epithelial cells

Get tips on using TriPure Isolation Reagent to perform RNA isolation / purification Tissue - Mouse Muscle

Get tips on using TriPure Isolation Reagent to perform RNA isolation / purification Tissue - Mouse Liver

Get tips on using EasySep™ Human T Cell Isolation Kit to perform Cell Isolation Human T cells

Get tips on using SV Total RNA Isolation System to perform

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.