siRNA / miRNA gene silencing Mouse MLO‐Y4

Get tips on using PE Mouse Anti-Human CD27 to perform Flow cytometry Anti-bodies Human - CD27

Get tips on using BV421 Mouse Anti-Human CD27 to perform Flow cytometry Anti-bodies Human - CD27

Get tips on using PE Mouse anti-Human CD105 to perform Flow cytometry Anti-bodies Human - CD105

Get tips on using FITC Mouse Anti-Human CD71 to perform Flow cytometry Anti-bodies Human - CD71

Get tips on using APC Mouse Anti-Human CD71 to perform Flow cytometry Anti-bodies Human - CD71

Get tips on using FITC Mouse Anti-Human CD90 to perform Flow cytometry Anti-bodies Human - CD90

Get tips on using PE Mouse Anti-Human CD90 to perform Flow cytometry Anti-bodies Human - CD90

Get tips on using BV605 Mouse Anti-Human CD15 to perform Flow cytometry Anti-bodies Human - CD15

Get tips on using PE Mouse Anti-Human CD44 to perform Flow cytometry Anti-bodies Human - CD44

DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.