Wound healing assay cell type rat

Get tips on using PE-Cy™7 Rat anti-Mouse CD117 to perform Flow cytometry Anti-bodies Mouse - CD117/c-kit

Get tips on using PE-Cy™7 Rat Anti-Mouse CD31 to perform Flow cytometry Anti-bodies Mouse - CD31/Pecam-1

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

Get tips on using Click-iT™ EdU Alexa Fluor™ 555 Imaging Kit to perform Cell cytotoxicity / Proliferation assay cell type - PC-3

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.

Get tips on using Autophagy Inhibitor, 3-MA to perform Autophagy assay cell type - Human fetal osteoblastic (hFOB) 1.19

Get tips on using LC3B Antibody Kit for Autophagy to perform Autophagy assay cell type - Normal human fibroblasts (NHFs)

Get tips on using Anti-Beclin 1 (Human) pAb to perform Autophagy assay cell type - Mouse white adipose tissue

Get tips on using Anti-SQSTM1 / p62 antibody (ab56416) to perform Autophagy assay cell type - MEFs (mouse embryonic fibroblasts)