siRNA / miRNA gene silencing Mouse 3T3-SA

Get tips on using Mouse GDNF ELISA Kit (ab171178) to perform ELISA Human - GDNF

Get tips on using Human/Mouse BDNF DuoSet ELISA to perform ELISA Human - BDNF

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.

Get tips on using Human/Mouse/Rat/Canine ALCAM/CD166 Antibody to perform Immunohistochemistry Mouse - CD166 / ALCAM

Get tips on using Mouse TNFSF11/RANKL PicoKine™ ELISA Kit to perform ELISA Mouse - RANK L

Get tips on using Mouse TNF Alpha PicoKine™ ELISA Kit to perform ELISA Mouse - TNF-alpha

Get tips on using Mouse Lipocalin-2 ELISA Kit (NGAL) (ab199083) to perform ELISA Mouse - NGAL/LCN2

Get tips on using Mouse KIM1 ELISA Kit (TIM-1) (ab119596) to perform ELISA Mouse - KIM-1

Get tips on using Mouse IGF-1 PicoKine™ ELISA Kit to perform ELISA Mouse - IGF-I

Get tips on using Mouse Heme Oxygenase 1 ELISA Kit (ab204524) to perform ELISA Mouse - HO-1