ChIP H3K27me3 Mouse Bovine Agarose

Get tips on using CD137 (4-1BB) Monoclonal Antibody (17B5), APC, eBioscience™ to perform Flow cytometry Anti-bodies Mouse - CD137

Get tips on using CD137 (4-1BB) Monoclonal Antibody (17B5), PE, eBioscience™ to perform Flow cytometry Anti-bodies Mouse - CD137

Get tips on using CD115 (c-fms) Monoclonal Antibody (AFS98), Biotin, eBioscience™ to perform Flow cytometry Anti-bodies Mouse - CD115

Get tips on using CD115 (c-fms) Monoclonal Antibody (AFS98), APC, eBioscience™ to perform Flow cytometry Anti-bodies Mouse - CD115

Get tips on using CD86 (B7-2) Monoclonal Antibody (GL1), APC, eBioscience™ to perform Flow cytometry Anti-bodies Mouse - CD86

Get tips on using CD8a Monoclonal Antibody (53-6.7), eFluor 450, eBioscience™ to perform Flow cytometry Anti-bodies Mouse - CD8a

Get tips on using CD11b Monoclonal Antibody (M1/70), eFluor 450, eBioscience™ to perform Flow cytometry Anti-bodies Mouse - CD11b

Get tips on using CD45 Monoclonal Antibody (30-F11), PE-Cyanine7, eBioscience™ to perform Flow cytometry Anti-bodies Mouse - CD45

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary mouse tracheal epithelial cells