shRNA gene silencing Mouse 4T1

Get tips on using Mouse/Rat FGF-21 Quantikine ELISA Kit to perform ELISA Rat - FGF-21

Get tips on using Rat/Mouse Cytochrome c Quantikine ELISA Kit to perform ELISA Rat - Cytochrome c

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

Get tips on using Purified anti-mouse CD274 (B7-H1, PD-L1) Antibody to perform Flow cytometry Anti-bodies Mouse - CD274/PD-L1

Get tips on using APC anti-mouse CD274 (B7-H1, PD-L1) Antibody to perform Flow cytometry Anti-bodies Mouse - CD274/PD-L1

Get tips on using PE anti-mouse CD273 (B7-DC, PD-L2) Antibody to perform Flow cytometry Anti-bodies Mouse - CD273/PD-L2

Get tips on using anti-alpha-Smooth Muscle Actin mouse monoclonal, ASM-1 to perform Immunohistochemistry Alpha smooth muscle Actin - Mouse -NA- -NA-

Get tips on using Monoclonal Anti-ACTA2 antibody produced in mouse to perform Western blotting Smooth muscle actin

Though DNA quantification is but one small step in the multifaceted DNA sample preparation workflow, it can have large implications on the performance and validity of conclusions drawn from downstream assays. Major challenges include accuracy, precision, reproducibility, and detection of present contamination. Among UV spectrophotometry, fluorescence and real-time PCR based methods, the quantification method should be chosen based on the requirement of the downstream assay .